PDB-3j02: Lidless D386A Mm-cpn in the pre-hydrolysis ATP-bound state

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 3j02
Title	Lidless D386A Mm-cpn in the pre-hydrolysis ATP-bound state
Descriptor	Lidless D386A Mm-cpn variant
Keywords	CHAPERONE, Mm-cpn, Chaperonin, ATP-bound
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Sequence details	AUTHORS STATE THAT THE LIDLESS MM-CPN IS A MUTANT THAT THE SEQUENCE (I241-K267) HAS BEEN REPLACED BY A SHORT LINKER (ETASE)
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with EM 3D map: EMDB-5258, Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-5258 Fit PDB-3j03 Fit: target map of fitting
Similar strucutres (beta)	Diagram EMDB-5140 PDB-3iyf PDB-4a0v EMDB-1961 EMDB-1397 List of similar structure data about Omokage system

Article
Citation - primary
Article	Structure, Vol. 19, Page: 633 - 639, Year: 2011,
Title	Cryo-EM structure of a group II chaperonin in the prehydrolysis ATP-bound state leading to lid closure.
Authors	Zhang, J., Ma, B., DiMaio, F., Douglas, N.R., Joachimiak, L.A., Baker, D., Frydman, J., Levitt, M., Chiu, W.
Links	DOI: 10.1016/j.str.2011.03.005

Components
ID 1 : Lidless D386A Mm-cpn variant
Image
Description	Lidless D386A Mm-cpn variant
Type	polypeptide(L)
Fragment	Lidless Mm-cpn
Mutation	D386A
Formula weight	52575.027 Da
Number of molecules	16
ID	1
Source	Method: Isolated from a genetically manipulated source Gene: ID:39152, Methanococcus maripaludis Host: ID:562, Escherichia coli
Links	UniProt: Q877G8, UniProt: Q877G8, Sequence view

Sample
Assembly
Aggregation state	PARTICLE
Experimental Mass	1
Theoretical Mass	1
Name	Lidless D386A Mm-cpn variant
Num components	1
Entity assembly
Assembly-ID	1
Name	Methanococcus maripaludis chaperonin
Type	PROTEIN
Experiment
Reconstruction method	SINGLE PARTICLE
Specimen type	VITREOUS ICE (CRYO EM)
Vitrification
Cryogen name	ETHANE
Humidity	100
Instrument	vitribot
Experiment
Method	ELECTRON MICROSCOPY

Electron Microscopy
Imaging
Microscope	Model: OTHER
Electron gun
Electron source	FIELD EMISSION GUN
Accelerating voltage	200 kV
Electron dose	20 e/A**2
Illumination mode	FLOOD BEAM
Lens
Mode	BRIGHT FIELD
Magnification	Calibrated: 112000 X, Nominal: 80000 X
Detector distance	0.0
Nominal defocus	Max: 2500 nm, Min: 1000 nm
Energy filter	in-column omega energy filter
Energy window	0-10 eV
Specimen holder
Specimen holder	Model: GATAN LIQUID NITROGEN, Type: Gatan side entry
Tilt angle	Min: 0.0 degrees, Max: 0.0 degrees
Detector
Type	Gatan 4Kx4K CCD camera