3D Electron Microscopy (3D-EM) Data Navigator [English / 日本語]
Fitting of influenza virus NP structure into the 9-fold symmetryzed cryoEM reconstruction of an active RNP particle.
by single particle reconstruction, at 12 A resolution
#1: Biological unit as monomeric, Made by Jmol
#2: Biological unit as monomeric, Made by Jmol
#3: Biological unit as monomeric, Made by Jmol
#4: Biological unit as monomeric, Made by Jmol
#5: Biological unit as monomeric, Made by Jmol
#6: Biological unit as monomeric, Made by Jmol
#7: Biological unit as monomeric, Made by Jmol
#8: Biological unit as monomeric, Made by Jmol
#9: Biological unit as monomeric, Made by Jmol
#10: Depositted structure unit, Made by Jmol
#11: Superimposing with EM 3D map: EMDB-1603, Made by UCSF CHIMERA
 Entry | |
| Summary | |
| Database / ID | PORTEIN DATA BANK (PDB) / 2wfs |
|---|---|
| Title | Fitting of influenza virus NP structure into the 9-fold symmetryzed cryoEM reconstruction of an active RNP particle. |
| Descriptor | NUCLEOPROTEIN |
| Keywords |  VIRAL PROTEIN, VIRAL NUCLEOPROTEIN, HOST-VIRUS INTERACTION, RNA VIRUSES, NUCLEOPROTEIN, RIBONUCLEOPROTEIN, RNA, VIRION, NUCLEUS, INFLUENZA, RNA-BINDING |
| PDBj Mine pages |  Summary, Structural Details, Experimental Details, Functional Details |
| Other databases | RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST |
| Structure Visualization | |
| Movies |  Movie Page#1: Biological unit as monomeric, Made by Jmol #2: Biological unit as monomeric, Made by Jmol #3: Biological unit as monomeric, Made by Jmol #4: Biological unit as monomeric, Made by Jmol #5: Biological unit as monomeric, Made by Jmol #6: Biological unit as monomeric, Made by Jmol #7: Biological unit as monomeric, Made by Jmol #8: Biological unit as monomeric, Made by Jmol #9: Biological unit as monomeric, Made by Jmol #10: Depositted structure unit, Made by Jmol #11: Superimposing with EM 3D map: EMDB-1603, Made by UCSF CHIMERA |
| Structure viewers |  Yorodumi, jV4, Jmol,  Biological unit (Images, jV) |
| Related Structure Data | |
| Related Entries |
Fit: target map of fitting |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - primary | |
| Article | Plos Pathog., Vol. 5, Page: 00491 - , Year: 2009, |
|---|---|
| Title | The Structure of a Biologically Active Influenza Virus Ribonucleoprotein Complex. |
| Authors | Coloma, R., Valpuesta, J.M., Arranz, R., Carrascosa, J.L., Ortin, J., Martin-Benito, J. |
| Links | DOI: 10.1371/JOURNAL.PPAT.1000491 |
 Components | |
| ID 1 : NUCLEOCAPSID PROTEIN, PROTEIN N | |
| Image |   |
|---|---|
| Description | NUCLEOPROTEIN |
| Type | polypeptide(L) |
| Formula weight | 56806.586 Da |
| Number of molecules | 9 |
| ID | 1 |
| Source | Method: Isolated from a genetically manipulated source Gene: A/VICTORIA/3/75 (H3N2), NP, ID:11320, INFLUENZA A VIRUSHost: AFRICAN GREEN MONKEY, ID:9534, KIDNEY, CHLOROCEBUS AETHIOPS |
| Links | Sequence view |
 Sample | |
| Assembly | |
| Aggregation state | PARTICLE |
|---|---|
| Details | MICROGRAPHS SELECTED BY COMPUTED CTF DATA AQUISITION |
| Name | INFLUENZA VIRUS RIBONUCLEOPROTEIN PARTICLE |
| Buffer | |
| Name | 50MM TRIS-HCL,100MM KCL, 5MM MGCL2,0.5% IGEPAL, 150MM IMIDAZOLE |
| Experiment | |
| Reconstruction method | SINGLE PARTICLE |
| Specimen type | VITREOUS ICE CRYO EM |
| Sample preparation | |
| pH | 8.0 |
| Sample support | |
| Details | HOLEY CARBON |
| Vitrification | |
| Details | CRYOGEN - ETHANE INSTRUMENT - LEICA PLUNGER |
| Experiment | |
| Method | ELECTRON MICROSCOPY |
 Electron Microscopy | |
| Imaging | |
| Microscope | Model: FEI TECNAI G2 |
|---|---|
| Electron gun | |
| Electron source | FIELD EMISSION GUN |
| Accelerating voltage | 200 kV |
| Illumination mode | FLOOD BEAM |
| Lens | |
| Mode | BRIGHT FIELD |
| Cs | Nominal: 2.26 mm |
| Specimen holder | |
| Temperature | 99 Kelvin |
| Detector | |
| Type | FILM (KODAK SO-136) |
| Image scans | |
| Number digital images | 159 |
 Processing | |
| 2D projection selection | |
| Number of particles | 9571 |
|---|---|
| Software name | SPIDER |
| Single particle entity | |
| Symmetry type | MIXED SYMMETRY |
| 3D reconstruction | |
| Actual pixel size | 2.8 A/pix |
| CTF correction method | WHOLE PLATE |
| Method | ANGULAR RECONSTITUTION,ITERATIVE ALGEBRAIC RECONSTRUCTION |
| Resolution | 12 A |
| 3D reconstruction | |
| Details | SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1603. |
| 3D fitting | |
| Method | RIGID BODY |
| Refinement Space | RECIPROCAL |
| Target criteria | VOLUMETRIC |
| 3D fitting list | |
| 3D fitting id | 1 |
| PDB entry ID | 2IQH |
| Refine | |
| Refine id | ELECTRON MICROSCOPY |
| Ls d res high | 12.00 A |
| Refine hist | |
| Cycle id | LAST |
| Refine id | ELECTRON MICROSCOPY |
| D res high | 12.00 |
| Total atoms | 15336 |
| Protein atoms | 15336 |
Top
Gallery
List
Diagram
Statistics
Documents
PDBj
EM Navigator
Detail page - PDB-2wfs
Download
Contact