PDB-2c7d: FITTED COORDINATES FOR GROEL-ADP7-GROES CRYO-EM COMPLEX...

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 2c7d
Title	FITTED COORDINATES FOR GROEL-ADP7-GROES CRYO-EM COMPLEX (EMD-1181)
Descriptor	60 KDA CHAPERONIN 10 KDA CHAPERONIN MOLECULE: GROES PROTEIN CPN10 GROES PROTEIN
Keywords	CHAPERONE, ATP-BINDING, ATOMIC STRUCTURE FITTING, CELL CYCLE, CELL DIVISION, CHAPERONIN, NUCLEOTIDE-BINDING, PHOSPHORYLATION
Authors	Ranson, N.A., Clare, D.K., Farr, G.W., Houldershaw, D., Horwich, A.L., Saibil, H.R.
Date	Deposition: 2005-11-22, Release: 2006-01-25
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with EM 3D map: EMDB-1181, Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-1181 CiteFit EMDB-1180 Cite PDB-2c7c Cite PDB-1aon PDB-1dk7 PDB-1dkd PDB-1egs PDB-1fy9 PDB-1fya PDB-1gr5 PDB-1grl PDB-1gru PDB-1j4z PDB-1jon PDB-1kid PDB-1kp8 PDB-1kpo PDB-1la1 PDB-1mnf PDB-1oel PDB-1pcq PDB-1pf9 PDB-1ss8 PDB-1svt PDB-1sx3 PDB-1sx4 PDB-1xck PDB-2c7e PDB-2cgt PDB-4aaq PDB-4aar PDB-4aas PDB-4aau PDB-4ab2 PDB-4ab3 Cite: data citing same article Fit: target map of fitting
Similar strucutres (beta)	Diagram PDB-2c7c PDB-2cgt EMDB-1180 PDB-1gru EMDB-1531 EMDB-1181 List of similar structure data about Omokage system

Article
Citation - primary
Article	Nat. Struct. Mol. Biol., Vol. 13, Issue 2, Page 147-52, Year 2006
Title	Allosteric signaling of ATP hydrolysis in GroEL-GroES complexes.
Authors	Neil A Ranson, Daniel K Clare, George W Farr, David Houldershaw, Arthur L Horwich, Helen R Saibil Astbury Centre for Structural Molecular Biology and Institute of Molecular & Cellular Biology, University of Leeds, Leeds, LS2 9JT, UK.
Keywords	Adenosine Diphosphate (chemistry, 58-64-0), Adenosine Triphosphate (metabolism, 56-65-5), Allosteric Regulation, Chaperonin 10 (chemistry), Chaperonin 60 (chemistry), Cryoelectron Microscopy, Hydrolysis, Models, Molecular, Multiprotein Complexes (chemistry), Protein Binding, Protein Structure, Quaternary
Links	DOI: 10.1038/nsmb1046, PubMed: 16429154, PMC: PMC2871290

Components
ID 1 : GROEL, PROTEIN CPN60, GROEL PROTEIN
Image
Description	60 KDA CHAPERONIN
Type	polypeptide(L)
Formula weight	57261.059 Da
Number of molecules	14
ID	1
Source	Method: Isolated from a genetically manipulated source Gene: ID:562, ESCHERICHIA COLI Host: ID:562, ESCHERICHIA COLI
Links	UniProt: P0A6F5, Sequence view
ID 2 : 10 KDA CHAPERONIN MOLECULE: GROES, PROTEIN CPN10, GROES PROTEIN
Image
Description	10 KDA CHAPERONIN MOLECULE: GROES, PROTEIN CPN10, GROES PROTEIN
Type	polypeptide(L)
Formula weight	10401.031 Da
Number of molecules	7
ID	2
Source	Method: Isolated from a genetically manipulated source Gene: ID:562, ESCHERICHIA COLI Host: ID:562, ESCHERICHIA COLI
Links	UniProt: P0A6F9, Sequence view

Processing
2D projection selection
Number of particles	7591
Software name	SPIDER, IMAGIC
Single particle entity
Symmetry type	CYCLIC
3D reconstruction
CTF correction method	FULL CORRECTION ON 2D CLASS AVERAGES
Details	RECIPROCAL SPACE FITTING OF SEVEN SEVEN INDEPENDENT RIGID BODIES WITH URO. FITTED ENTITIES WERE GROEL EQUATORIAL (RESIDUES 3-136 AND 410-524), INTERMEDIATE (RESIDUES 137-192 AND 374-409) AND APICAL (RESIDUES 192-373) DOMAINS, PLUS A GROES SUBUNIT. THE MAP INTO WHICH THESE COORDINATES WERE FITTED IS AVAILABLE AT THE EMD (EMD-1181)
Method	PROJECTION MATCHING-BASED ANGULAR REFINEMENT OF MSA GENERATED CLASSES. ITERATIVE ALGEBRAIC RECONSTRUCTION IN SPIDER.
Nominal pixel size	1.4 A/pix
Resolution	8.7 A
3D fitting
Method	RECIPROCAL SPACE FITTING IN URO
Refine
Refine id	ELECTRON MICROSCOPY
Ls d res high	8.70 A
Refine hist
Cycle id	LAST
Refine id	ELECTRON MICROSCOPY
D res high	8.70
Total atoms	57946
Protein atoms	57946