 Entry |
| Summary |
| Database / ID | EM DATA BANK (EMDB) / 5371 |
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| Title | Cryo-EM structure of full-length NSF in the ADP state
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| Map | NSF hexamer in the ADP state |
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| Sample | N-ethylmaleimide-sensitive factor (NSF) |
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| Keywords |  membrane fusion, AAA+ ATPase |
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| Authors | Chang LF, Chen S, Liu CC, Pan X, Jiang J, Bai XC, Xie X, Wang HW, Sui SF |
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| Date | Deposition: 2011-12-15, Header release: 2012-01-03, Map release: 2012-02-01, Last update: 2012-02-01 |
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| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
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| Structure Visualization |
| Movies |  Movie Page
#1: Surface view with section colored by density value, Surface level: 3.5, Made by UCSF CHIMERA #2: Surface view colored by cylindrical radius, Surface level: 3.5, Made by UCSF CHIMERA |
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| Supplemental images |
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| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
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| Related Structure Data |
| Related Entries |
Cite: data citing same article |
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| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
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 Article |
| Citation - Primary |
| Article | Nat. Struct. Mol. Biol., Vol. 19, Issue 3, Page 268-75, Year 2012 |
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| Title | Structural characterization of full-length NSF and 20S particles. |
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| Authors | Lei-Fu Chang, Song Chen, Cui-Cui Liu, Xijiang Pan, Jiansen Jiang, Xiao-Chen Bai, Xin Xie, Hong-Wei Wang, Sen-Fang Sui
State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China. |
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| Keywords | Adaptor Proteins, Vesicular Transport (chemistry), Adenosine Diphosphate (chemistry, 58-64-0), Animals, Cricetinae, Cricetulus, Cryoelectron Microscopy, Models, Molecular, N-Ethylmaleimide-Sensitive Proteins (chemistry, 3.6.4.6), Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary, SNARE Proteins (chemistry), Structural Homology, Protein, Substrate Specificity |
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| Links | DOI: 10.1038/nsmb.2237, PubMed: 22307055 |
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 Map |
| File |  EMD-5371.map ( map file in CCP4 format, 8390 KB ) |
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| Projections & Slices | Size of images: | Axes |  Z (Sec.) |  Y (Row.) |  X (Col.) |
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| Surface |  |  |  |
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Projections |  |  |  |
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Slices (1/3) |  |  |  |
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Slices (1/2) |  |  |  |
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Slices (2/3) |  |  |  |
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Images are generated by Spider package. |
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| Density |
| Contour Level: | 2.6 (by author), 3.5 (movie #1): |
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| Minimum - Maximum: | -5.46910524 - 9.45040226 |
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| Average (Standard dev.): | 0.14773960 (1.22013593) |
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|
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| Data Type | Image stored as Reals |
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| Space Group Number | 1 |
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| Map Geometry | | Axis Order : | X | Y | Z |
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| Dimensions : | 128 | 128 | 128 |
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| Origin : | -64 | -64 | -64 |
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| Limit : | 63 | 63 | 63 |
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| Spacing : | 128 | 128 | 128 |
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|
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| Unit Cell | A = 230.4 A , B = 230.4 A , C = 230.4 A ,
alpha = 90.0 degrees , beta = 90.0 degrees , gamma = 90.0 degrees |
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| Pixel Spacing | X = 1.8 A , Y = 1.8 A , Z = 1.8 A |
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| CCP4 map header info | | mode | Image stored as Reals |
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| A/pix X/Y/Z | 1.8 | 1.8 | 1.8 |
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| M x/y/z | 128 | 128 | 128 |
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| origin x/y/z | 0.000 | 0.000 | 0.000 |
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| length x/y/z | 230.400 | 230.400 | 230.400 |
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| alpha/beta/gamma | 90.000 | 90.000 | 90.000 |
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| start NX/NY/NZ | -62 | -62 | -62 |
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| NX/NY/NZ | 125 | 125 | 125 |
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| MAP C/R/S | 1 | 2 | 3 |
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| start NC/NR/NS | 0 | 0 | 0 |
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| NC/NR/NS | 128 | 128 | 128 |
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| start NC,NX/NR,NY/NS,NZ | 0 | 0 | 0 |
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| NC,NX/NR,NY/NS,NZ | 128 | 128 | 128 |
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| D min/max/mean | -5.469 | 9.450 | 0.148 |
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| Annotation Details | NSF hexamer in the ADP state |
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