EntrySummary Database / ID EM DATA BANK (EMDB) / 5324 Title Molecular structure of soluble trimeric HIV-1 glycoprotein gp140 JR-FL with sCD4 and 17b Map Molecular structure of soluble trimeric HIV-1 glycoprotein gp140 JR-FL with sCD4 and 17b Sample SOSIP gp140 JR-FL sCD4 17b Keywords HIV/AIDS vaccine , immunogen , HIV glycoprotein structure , gp140 trimer , cryo-electron tomography , sub-tomogram averaging , cryo-electron microscopyAuthors Harris A , Borgnia MJ , Shi D , Bartesaghi A , He H , Pejchal R , Kang YK , Depetris R , Marozsan AJ , Sanders RW , Klasse PJ , Milne JL , Wilson IA , Olson WC , Moore JP , Subramaniam S Date Deposition : 2011-07-11, Header release : 2012-05-31, Map release : 2012-05-31, Last update : 2012-05-31EMDB Sites EMDB @PDBe (EU) , EMDB @RCSB (USA)Structure Visualization Movies Play Pause Small Medium LargeX OffMovie Page #1 #2 #1 : Surface view with section colored by density value, Surface level: 1.45, Made by UCSF CHIMERA
#2 : Surface view colored by radius, Surface level: 1.45, Made by UCSF CHIMERA
Supplemental images
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Similar strucutres (beta)
List of similar structure data about Omokage system
ArticleCitation - Primary Article Proc. Natl. Acad. Sci. U.S.A. , Vol. 108, Issue 28, Page 11440-5, Year 2011Title Trimeric HIV-1 glycoprotein gp140 immunogens and native HIV-1 envelope glycoproteins display the same closed and open quaternary molecular architectures. Authors Audray Harris , Mario J Borgnia , Dan Shi , Alberto Bartesaghi , Haifeng He , Robert Pejchal , Yun Kenneth Kang , Rafael Depetris , Andre J Marozsan , Rogier W Sanders , Per Johan Klasse , Jacqueline L S Milne , Ian A Wilson , William C Olson , John P Moore , Sriram Subramaniam Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.Keywords AIDS Vaccines (chemistry), Antigens, CD4 (chemistry), Biophysical Phenomena , Cryoelectron Microscopy , HIV Antibodies (chemistry), HIV Antigens (chemistry), HIV Envelope Protein gp120 (chemistry), HIV-1 (chemistry), Humans , Immunoglobulin Fab Fragments (chemistry), Ligands , Models, Molecular , Protein Structure, Quaternary , env Gene Products, Human Immunodeficiency Virus (chemistry), gp120 protein, Human immunodeficiency virus 1 , gp140 envelope protein, Human immunodeficiency virus 1Links DOI: 10.1073/pnas.1101414108 , PubMed: 21709254 , PMC: PMC3136299
MapFile EMD-5324.map ( map file in CCP4 format, 4002 KB )Projections & Slices Size of images: Small Medium LargeAxes Z (Sec.) Y (Row.) X (Col.)X SurfaceX ProjectionsX Slices (1/3)X Slices (1/2)X Slices (2/3)
Images are generated by Spider package .
Density
Contour Level: 1.45 (by author) , 1.45 (movie #1): Minimum - Maximum: -7.14025879 - 10.19021034 Average (Standard dev.): -0.00029482 (0.70859784)
Data Type Image stored as Reals Space Group Number 1 Map Geometry Axis Order : X Y Z Dimensions : 100 100 100 Origin : 0 0 0 Limit : 99 99 99 Spacing : 100 100 100
Unit Cell A = 410.0 A , B = 410.0 A , C = 410.0 A , alpha = 90.0 degrees , beta = 90.0 degrees , gamma = 90.0 degreesPixel Spacing X = 4.1 A , Y = 4.1 A , Z = 4.1 ACCP4 map header info Show mode Image stored as Reals A/pix X/Y/Z 4.1 4.1 4.1 M x/y/z 100 100 100 origin x/y/z 0.000 0.000 0.000 length x/y/z 410.000 410.000 410.000 alpha/beta/gamma 90.000 90.000 90.000 start NX/NY/NZ NX/NY/NZ MAP C/R/S 1 2 3 start NC/NR/NS 0 0 0 NC/NR/NS 100 100 100 start NC,NX/NR,NY/NS,NZ 0 0 0 NC,NX/NR,NY/NS,NZ 100 100 100 D min/max/mean -7.140 10.190 -0.000
Annotation Details Molecular structure of soluble trimeric HIV-1 glycoprotein gp140 JR-FL with sCD4 and 17b