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negative stain Electron Microscopy of the N-Ethylmaleimide Sensitive Factor (NSF)
by single particle reconstruction
#1: Surface view with section colored by density value, Surface level: 0.012, Made by UCSF CHIMERA
#2: Surface view colored by cylindrical radius, Surface level: 0.012, Made by UCSF CHIMERA
 Entry | |
| Summary | |
| Database / ID | EM DATA BANK (EMDB) / 2041 |
|---|---|
| Title | negative stain Electron Microscopy of the N-Ethylmaleimide Sensitive Factor (NSF) |
| Map | NSF-D1D2 |
| Sample | NSF-D1D2 |
| Keywords |  NSF, N-Ethylmaleimide Sensitive Factor |
| Authors | Moeller A, Zhao C, Fried MG, Wilson-Kubalek EM, Carragher B, Whiteheart SW |
| Date | Deposition: 2012-01-23, Header release: 2012-02-15, Map release: 2012-02-15, Last update: 2012-02-15 |
| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
| Structure Visualization | |
| Movies |  Movie Page#1: Surface view with section colored by density value, Surface level: 0.012, Made by UCSF CHIMERA #2: Surface view colored by cylindrical radius, Surface level: 0.012, Made by UCSF CHIMERA |
| Supplemental images | |
| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
| Related Structure Data | |
| Related Entries |
Cite: data citing same article |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - Primary | |
| Article | J. Struct. Biol., Vol. 177, Issue 2, Page 335-43, Year 2012 |
|---|---|
| Title | Nucleotide-dependent conformational changes in the N-Ethylmaleimide Sensitive Factor (NSF) and their potential role in SNARE complex disassembly. |
| Authors | Arne Moeller, Chunxia Zhao, Michael G Fried, Elizabeth M Wilson-Kubalek, Bridget Carragher, Sidney W Whiteheart The Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA. |
| Keywords | Adenine Nucleotides (chemistry), Amino Acid Substitution, Animals, CHO Cells, Cricetinae, Microscopy, Electron, Models, Molecular, N-Ethylmaleimide-Sensitive Proteins (chemistry, 3.6.4.6), Protein Binding, Protein Structure, Quaternary, Protein Structure, Tertiary, SNARE Proteins (metabolism), Surface Properties, Ultracentrifugation |
| Links | PII: S1047-8477(12)00002-0, DOI: 10.1016/j.jsb.2011.12.018, PubMed: 22245547, PMC: PMC3382979 |
 Map | |||||||||||||||||||||||||
| File |  EMD-2041.map ( map file in CCP4 format, 3540 KB ) | ||||||||||||||||||||||||
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| Projections & Slices | Size of images:
Images are generated by Spider package. | ||||||||||||||||||||||||
| Density |
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| Data Type | Image stored as Reals | ||||||||||||||||||||||||
| Space Group Number | 1 | ||||||||||||||||||||||||
| Map Geometry |
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| Unit Cell | A= B= C: 216.95999 A Alpha=beta=gamma: 90 degrees | ||||||||||||||||||||||||
| Pixel Spacing | X= Y= Z: 2.26 A | ||||||||||||||||||||||||
| CCP4 map header info | |||||||||||||||||||||||||
| Annotation Details | NSF-D1D2 | ||||||||||||||||||||||||
 Supplement | |
| Images | |
| Images | |
|---|---|
 Sample | |
| Name | NSF-D1D2 |
|---|---|
| Number of Components | 1 |
| Oligomeric State | hexamer |
| Component #1: protein - NSF | |
| Scientific name | N-Ethylmaleimide Sensitive Factor |
| Common Name | NSF |
| Oligomeric Details | hexamer |
| Scientific Name of Species | Cricetulus griseus |
| Common Name of Species | Chinese hamster |
| NCBI taxonomy | 10029 |
| Recombinant expression | Yes |
 Experiment | |
| Sample Preparation | |
| Specimen State | particle |
|---|---|
| Vitrification | |
| Cryogen Name | NONE |
| Instrument | NONE |
| Imaging | |
| Microscope | FEI TECNAI F20 |
| Date | 20-MAY-2011 |
| Electron Gun | |
| Electron Source | FIELD EMISSION GUN |
| Accelerating Voltage | 120 kV |
| Electron Dose | 25 e/A**2 |
| Illumination Mode | FLOOD BEAM |
| Lens | |
| Magnification | Nominal: 50000 |
| Imaging Mode | BRIGHT FIELD |
| Defocus | -200 nm - -2000 nm |
| Specimen Holder | |
| Model | SIDE ENTRY, EUCENTRIC |
| Camera | |
| Image Acquisition | |
| Number of Digital Images | 448 |
 Processing | |
| Method | single particle reconstruction |
|---|---|
| 3D reconstruction | |
| Software | Xmipp,Appion,Imagic |
| CTF Correction | whole image |
| Single Particle | |
| Number of Projections | 20104 |
| Download | |||
| Data from EMDB | |||
| Header (meta data in XML format) | emd-2041.xml (5.8 KB) | ||
|---|---|---|---|
| Map data | emd_2041.map.gz (2.2 MB) | ||
| Images |  emd_2041.jpg (275.1 KB) | ||
| FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-2041 | ||
| Movie files | |||
| movie #1 |
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| movie #2 |
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