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PDBj>EM Navigator>Detail page - EMDB-1821

Human anaphase promoting complex with substrate

by single particle reconstruction, at 28 A resolution

Movie

Orientation:

#1: Surface view with section colored by density value, Surface level: 0.08, Made by UCSF CHIMERA

#2: Surface view colored by radius, Surface level: 0.08, Made by UCSF CHIMERA

Entry
Summary
Database / IDEM DATA BANK (EMDB) / 1821
AuthorsBuschhorn BA, Petzold G, Galova M, Dube P, Kraft C, Herzog F, Peters JM, Stark H
EMDB SitesEMDB @PDBe (EU), EMDB @RCSB (USA)
Structure Visualization
MoviesMovie Page

#1: Surface view with section colored by density value, Surface level: 0.08, Made by UCSF CHIMERA

#2: Surface view colored by radius, Surface level: 0.08, Made by UCSF CHIMERA

Supplemental images
Structure viewersYorodumi, Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe)
Related Structure Data
Related Entries

Cite: data citing same article

Similar strucutres (beta)
List of similar structure data about Omokage system
Article
Citation - Primary
ArticleNat. Struct. Mol. Biol., Vol. 18, Issue 1, Page 6-13, Year 2011
TitleSubstrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1.
AuthorsBettina A Buschhorn, Georg Petzold, Marta Galova, Prakash Dube, Claudine Kraft, Franz Herzog, Holger Stark, Jan-Michael Peters
Research Institute of Molecular Pathology (IMP), Vienna, Austria.
KeywordsCDC16 protein, S cerevisiae, CDC27 protein, S cerevisiae (6.3.2.19), CDH1 protein, human, Cadherins (metabolism), Cell Cycle Proteins (metabolism), Cytokines (metabolism), DOC1 protein, S cerevisiae, DOC1 protein, human, Hct1 protein, S cerevisiae, Humans, Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae Proteins (metabolism), Ubiquitin-Protein Ligase Complexes (chemistry, 6.3.2.19), Ubiquitin-Protein Ligases (metabolism, 6.3.2.19), Ubiquitination, anaphase-promoting complex (6.3.2.19)
LinksDOI: 10.1038/nsmb.1979, PubMed: 21186364
Map
FileEMD-1821.map ( map file in CCP4 format, 4002 KB )
Projections & SlicesSize of images:
AxesZ (Sec.)Y (Row.)X (Col.)
Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Density
Contour Level:0.08 (by author), 0.08 (movie #1):
Minimum - Maximum: -0.49782348 - 0.81367147
Average (Standard dev.): -0.00016081 (0.07102545)
Data TypeImage stored as Reals
Space Group Number1
Map Geometry
Axis Order : X Y Z
Dimensions : 100 100 100
Origin : 0 0 0
Limit : 99 99 99
Spacing : 100 100 100
Unit CellA = 360.0 A , B = 360.0 A , C = 360.0 A ,
alpha =
90.0 degrees , beta = 90.0 degrees , gamma = 90.0 degrees
Pixel SpacingX = 3.6 A , Y = 3.6 A , Z = 3.6 A
CCP4 map header info
modeImage stored as Reals
A/pix X/Y/Z3.63.63.6
M x/y/z100100100
origin x/y/z0.0000.0000.000
length x/y/z360.000360.000360.000
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ-70-70-70
NX/NY/NZ140140140
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS100100100
start NC,NX/NR,NY/NS,NZ
NC,NX/NR,NY/NS,NZ
D min/max/mean-0.4980.814-0.000
Annotation Details3D structure of the human Anaphase Promoting Complex substrate complex.3.
Supplement
Images
Images
Sample
NameYeast anaphase promoting complex
Number of Components1
Component #1: cellular-component - APCC
Scientific nameAPCC
Oligomeric DetailsMonomer
Scientific Name of SpeciesSaccharomyces cerevisiae (NCBI Taxonomy: 4932)

Common Name of SpeciesBakers' yeast
Recombinant expressionNo
Experiment
Sample Preparation
Specimen Stateparticle
Vitrification
Cryogen NameNITROGEN
InstrumentNONE
Imaging
MicroscopeFEI/PHILIPS CM200FEG
Electron Gun
Electron SourceFIELD EMISSION GUN
Accelerating Voltage160 kV
Illumination ModeSPOT SCAN
Lens
Imaging ModeBRIGHT FIELD
Specimen Holder
HolderEucentric ( GATAN LIQUID NITROGEN )
Camera
Image Acquisition
Sampling Size1.8 microns
Processing
Methodsingle particle reconstruction
3 D reconstruction
Resolution By Author28
Download
Data from EMDB
Header (meta data in XML format)emd-1821.xml (5.4 KB)
Map dataemd_1821.map.gz (3.6 MB)
Imagesimage1821.png (367.8 KB)
FTP directoryftp://ftp.pdbj.org/pub/emdb/structures/EMD-1821
Movie files
movie #1
.mp4 (H.264/MPEG-4 AVC format), 3.6 MB
.webm (WebM/VP8 format), 5.5 MB
Session file for UCSF-Chimera, 26.7 KB
movie #2
.mp4 (H.264/MPEG-4 AVC format), 3.3 MB
.webm (WebM/VP8 format), 5.1 MB
Session file for UCSF-Chimera, 26.7 KB