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A mechanical explanation of RNA pseudoknot function in programmed ribosomal frameshifting.

by single particle reconstruction, at 14.0 A resolution

Movie

Orientation:

#1: Surface view with section colored by density value, Surface level: 27, Made by UCSF CHIMERA

#2: Surface view colored by height, Surface level: 27, Made by UCSF CHIMERA

Entry
Summary
Database / IDEM DATA BANK (EMDB) / 1199
AuthorsNamy O, Moran SJ, Stuart DI, Gilbert RJC, Brierley I
EMDB SitesEMDB @PDBe (EU), EMDB @RCSB (USA)
Structure Visualization
MoviesMovie Page

#1: Surface view with section colored by density value, Surface level: 27, Made by UCSF CHIMERA

#2: Surface view colored by height, Surface level: 27, Made by UCSF CHIMERA

Supplemental images
Structure viewersYorodumi, Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe)
Related Structure Data
Related Entries

Cite: data citing same article

Similar strucutres (beta)
List of similar structure data about Omokage system
Article
Citation - Primary
ArticleNature, Vol. 441, Issue 7090, Page 244-7, Year 2006
TitleA mechanical explanation of RNA pseudoknot function in programmed ribosomal frameshifting.
AuthorsOlivier Namy, Stephen J Moran, David I Stuart, Robert J C Gilbert, Ian Brierley
Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
KeywordsAnimals, Coronavirus (genetics), Frameshift Mutation (genetics), Frameshifting, Ribosomal (physiology), Models, Biological, Models, Molecular, Movement, Nucleic Acid Conformation, RNA, Messenger (chemistry), RNA, Transfer (chemistry, 9014-25-9), Rabbits, Ribosomes (chemistry)
LinksDOI: 10.1038/nature04735, PubMed: 16688178
Map
FileEMD-1199.map ( map file in CCP4 format, 16385 KB )
Projections & SlicesSize of images:
AxesZ (Sec.)X (Row.)Y (Col.)
Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider package.

Density
Contour Level:26.3, 27 (movie #1):
Minimum - Maximum: -152.78 - 200
Average (Standard dev.): -1.32511 (13.7607)
Data Typefloat (32-bit)
Space Group Number1
Map Geometry
Axis Order : Y X Z
Dimensions : 160 160 160
Origin : -80 -80 -79
Limit : 79 79 80
Spacing : 160 160 160
Unit CellA = 532.8 A , B = 532.8 A , C = 532.8 A ,
alpha =
90 degrees , beta = 90 degrees , gamma = 90 degrees
Pixel SpacingX = 3.33 A , Y = 3.33 A , Z = 3.33 A
CCP4 map header info
modeImage stored as Reals
A/pix X/Y/Z3.333.333.33
M x/y/z160160160
origin x/y/z0.0000.0000.000
length x/y/z532.800532.800532.800
alpha/beta/gamma90.00090.00090.000
start NX/NY/NZ-80-80-79
NX/NY/NZ160160160
MAP C/R/S213
start NC/NR/NS-80-80-79
NC/NR/NS160160160
start NC,NX/NR,NY/NS,NZ
NC,NX/NR,NY/NS,NZ
D min/max/mean-152.780200.000-1.325
Annotation DetailsReconstruction of rabbit reticulocyte ribosome, purified from Promega RRL lysate
Supplement
Images
Images
Sample
NameRabbit reticuloyte ribosome
Oligomeric StateOne each of 40S and 60S
Number of Components2
Experimental Mass3.5 MDa
Theoretical Mass3.5 MDa
Component #1: ribosome-eukaryote - 40S
Scientific nameSmall subunit
Common Name40S
Scientific Name of SpeciesOryctolagus cuniculus (NCBI Taxonomy: 9986)

Common Name of SpeciesRabbit
EukaryoteSSU 40S
Recombinant expressionNo
Component #2: ribosome-eukaryote - 60S
Scientific nameLarge subunit
Common Name60S
Scientific Name of SpeciesOryctolagus cuniculus (NCBI Taxonomy: 9986)
Common Name of SpeciesRabbit
EukaryoteLSU 60S
Recombinant expressionNo
Experiment
Sample Preparation
Specimen Support Details300 mesh gold w/ lacey carbon
Specimen Stateparticle
Vitrification
Cryogen NameETHANE
Imaging
MicroscopeFEI/PHILIPS CM200FEG
Electron Gun
Electron SourceFIELD EMISSION GUN
Accelerating Voltage200 kV
Electron Dose2 e/A**2
Illumination ModeSPOT SCAN
Lens
MagnificationNominal: 50000 X,
Nominal Cs2 mm
Imaging ModeBRIGHT FIELD
Defocus1000 nm - 7000 nm
Specimen Holder
HolderEucentric ( GATAN LIQUID NITROGEN )
Temperature100 Kelvin
Camera
DetectorKodak SO163 film
Image Acquisition
Number of Digital Images11
Sampling Size3.33 microns
Od Range5
Quant Bit Number8
ScannerOTHER
Processing
Methodsingle particle reconstruction
3 D reconstruction
AlgorithmSingle particle
SoftwareSPIDER and GAP
CTF CorrectionBy micrograph
Resolution By Author14.0
Resolution MethodFSC at 0.5 cut-off
Euler Angles DetailsSPIDER
DetailsDeposited map is composite of computationally-separated subunits.
Single Particle
Number of Projections10296
DetailsThe particles were selected in a semi-automated fashion using BOXER (EMAN suite)
Atomic Model Fitting
Model #0
Refinement Protocolrigid body
Target CriteriaCC
SoftwareURO
DetailsProtocol: Rigid body. Subunits manually docked in O and refined in URO
Download
Data from EMDB
Header (meta data in XML format)emd-1199.xml (7.5 KB)
Map dataemd_1199.map.gz (747 KB)
Images1199.gif (55.2 KB)
FTP directoryftp://ftp.pdbj.org/pub/emdb/structures/EMD-1199
Movie files
movie #1
.mp4 (H.264/MPEG-4 AVC format), 3.3 MB
.webm (WebM/VP8 format), 5.2 MB
Session file for UCSF-Chimera, 20.1 KB
movie #2
.mp4 (H.264/MPEG-4 AVC format), 3.2 MB
.webm (WebM/VP8 format), 4.5 MB
Session file for UCSF-Chimera, 20.2 KB