PDB-3zw6: MODEL OF HEXAMERIC AAA DOMAIN ARRANGEMENT OF GREEN-TYPE...

Entry
Summary
Database / ID	PORTEIN DATA BANK (PDB) / 3zw6
Title	MODEL OF HEXAMERIC AAA DOMAIN ARRANGEMENT OF GREEN-TYPE RUBISCO ACTIVASE FROM TOBACCO.
Descriptor	RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE 1 CHLOROPLASTIC
Keywords	PHOTOSYNTHESIS, NEGATIVE STAIN EM
PDBj Mine pages	Summary, Structural Details, Experimental Details, Functional Details
Other databases	RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST
Structure Visualization
Movies	Movie Page #1: Depositted structure unit, Made by Jmol #2: Superimposing with EM 3D map: EMDB-1940, Made by UCSF CHIMERA
Structure viewers	Yorodumi, jV4, Jmol, Biological unit (Images, jV)
Related Structure Data
Related Entries	EMDB-1940 Fit PDB-3t15 Fit: target map of fitting
Similar strucutres (beta)	Diagram PDB-2rec PDB-3zuh EMDB-1940 List of similar structure data about Omokage system

Article
Citation - primary
Article	Nat.Struct.Mol.Biol., Vol. 18, Page: 1366 - , Year: 2011,
Title	Structure of Green-Type Rubisco Activase from Tobacco
Authors	Stotz, M., Mueller-Cajar, O., Ciniawsky, S., Wendler, P., Hartl, F.U., Bracher, A., Hayer-Hartl, M.
Links	DOI: 10.1038/NSMB.2171

Components
ID 1 : RA 1, RUBISCO ACTIVASE 1
Image
Description	RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE 1, CHLOROPLASTIC
Type	polypeptide(L)
Fragment	AAA ATPASE DOMAIN, RESIDUES 127-419
Formula weight	32857.035 Da
Number of molecules	6
ID	1
Source	Method: Isolated from a genetically manipulated source Gene: COMMON TOBACCO, ID:4097, NICOTIANA TABACUM Host: ID:469008, ESCHERICHIA COLI , BL21(DE3) Plasmid name: PHUENTRCA
Links	UniProt: Q40460, Sequence view

Sample
Assembly
Aggregation state	PARTICLE
Details	THE PROTEIN FOR EM CARRIES A R294V MUTATION
Name	NICOTIANA TABACUM RUBISCO ACTIVASE (R294V)
Buffer
Name	20MM TRIS-HCL, 50MM NACL, 2MM MGCL2,1MM ATPGAMMAS
Experiment
Reconstruction method	SINGLE PARTICLE
Specimen type	NEGATIVE STAIN
Sample preparation
pH	8.0
Sample concentration	0.04 mg/ml
Sample support
Details	CARBON
Experiment
Method	ELECTRON MICROSCOPY

Processing
2D projection selection
Number of particles	599
Software name	MRC, IMAGIC, SPIDER
Single particle entity
Symmetry type	MIXED SYMMETRY
3D reconstruction
Actual pixel size	3.308 A/pix
CTF correction method	PHASE FLIPPING, EACH PARTICLE
Details	A MODULE OF ALPHA HELICAL DOMAIN AND ALPHA-BETA DOMAIN OF THE NEIGHBOURING SUBUNIT WAS OVERLAID WITH THE P97 D2 (3CF3) STRUCTURE AND THE HEXAMER WAS FITTED INTO THE EM MAP SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1940. (DEPOSITION ID: 10169).
Method	ANGULAR RECONSTITUTION
Nominal pixel size	3.308 A/pix
Resolution	20 A
3D fitting
Method	RIGID BODY
Refinement Protocol	X-RAY
Refinement Space	REAL
Target criteria	LOCAL MINIMISATION, FIT IN MAP
3D fitting list
3D fitting id	1
PDB entry ID	3T15 PDB-3t15
Refine
Refine id	ELECTRON MICROSCOPY
Ls d res high	20.00 A
Refine hist
Cycle id	LAST
Refine id	ELECTRON MICROSCOPY
D res high	20.00
Total atoms	12492
Protein atoms	12492