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Model of actin-fimbrin ABD2 complex
by helical reconstruction, at 12 A resolution
 Entry | |
| Summary | |
| Database / ID | PORTEIN DATA BANK (PDB) / 3byh |
|---|---|
| Title | Model of actin-fimbrin ABD2 complex |
| Descriptor | Actin fimbrin ABD2 |
| Keywords |  STRUCTURAL PROTEIN, helical filament, protein polymer, ATP-binding, Cytoskeleton, Methylation, Nucleotide-binding, Phosphoprotein, Structural protein |
| PDBj Mine pages |  Summary, Structural Details, Experimental Details, Functional Details |
| Other databases | RCSB-PDB, PDBe, CATH, CE, FSSP, SCOP, VAST |
| Structure Visualization | |
| Movies |  Movie Page#1: Biological unit as representative helical assembly, Made by Jmol #2: Depositted structure unit, Made by Jmol |
| Structure viewers |  Yorodumi, jV4, Jmol,  Biological unit (Images, jV) |
| Related Structure Data | |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - primary | |
| Article | Proc.Natl.Acad.Sci.Usa, Vol. 105, Page: 1494 - 1498, Year: 2008, |
|---|---|
| Title | High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex. |
| Authors | Galkin, V.E., Orlova, A., Cherepanova, O., Lebart, M.C., Egelman, E.H. |
| Links | DOI: 10.1073/pnas.0708667105 |
 Components | |
| ID 1 : Beta-actin, PS1TP5-binding protein 1 | |
| Image |   |
|---|---|
| Description | Actin |
| Type | polypeptide(L) |
| Formula weight | 41651.809 Da |
| Number of molecules | 1 |
| ID | 1 |
| Source | Method: Isolated from a genetically manipulated source Gene: human, Homo, PS1TP5BP1, ID:9606, Homo sapiensHost: Escherichia, ID:562, Escherichia coli
|
| Links | UniProt: Q1KLZ0, Sequence view |
| ID 2 : fimbrin ABD2 | |
| Image |   |
| Description | fimbrin ABD2 |
| Type | polypeptide(L) |
| Formula weight | 26764.547 Da |
| Number of molecules | 1 |
| ID | 2 |
| Source | Method: Isolated from a genetically manipulated source Gene: human, Homo, ID:9606, Homo sapiensHost: Escherichia, ID:562, Escherichia coli |
| Links | Sequence view |
 Sample | |
| Assembly | |
| Aggregation state | FILAMENT |
|---|---|
| Entity assembly | |
| Assembly-ID | 1 |
| Details | rotation by -166.5 degrees about the z-axis (x=0, y=0), translation by 27.3 Angstroms along the z-axis |
| Name | helical filament |
| Experiment | |
| Reconstruction method | HELICAL |
| Specimen type | VITREOUS ICE (CRYO EM) |
| Experiment | |
| Method | ELECTRON MICROSCOPY |
 Electron Microscopy |
 Processing | |
| 3D reconstruction | |
| Actual pixel size | 2.38 A/pix |
|---|---|
| CTF correction method | micrographs were multiplied by the CTF function, and the final reconstruction was then divided by the summed squared CTFs |
| Magnification calibration | TMV |
| Method | IHRSR |
| Nominal pixel size | 2.38 A/pix |
| Resolution | 12.0 A |
| 3D fitting list | |
| 3D fitting id | 1 |
| Refine hist | |
| Cycle id | LAST |
| Refine id | ELECTRON MICROSCOPY |
| Total atoms | 4795 |
| Protein atoms | 4795 |
 Download | |||
| PDB format | |||
| All | pdb3byh.ent.gzpdb3byh.ent (uncompressed file) | ||
|---|---|---|---|
| Header only | pdb3byh.ent.gz | ||
| mmCIF format | |||
| mmCIF | 3byh.cif.gz | ||
| XML format | |||
| All | 3byh.xml.gz | ||
| No-atom | 3byh-noatom.xml.gz | ||
| Ext-atom | 3byh-extatom.xml.gz | ||
| Movie files | |||
| movie #1 |
| ||
| movie #2 |
| ||
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