EMDB-1979: Structural Dynamics of Archaeal Small Heat Shock Proteins

Entry
Summary
Database / ID	EM DATA BANK (EMDB) / 1979
Authors	Haslbeck M, Kastenmueller A, Buchner J, Weinkauf S, Braun N
EMDB Sites	EMDB @PDBe (EU), EMDB @RCSB (USA)
Structure Visualization
Movies	Movie Page #1: Surface view with section colored by density value, Surface level: 0.008, Made by UCSF CHIMERA #2: Surface view colored by radius, Surface level: 0.008, Made by UCSF CHIMERA
Supplemental images	Hsp16.5_smal...
Structure viewers	Yorodumi, Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe)
Related Structure Data
Related Entries	EMDB-1889 Cite EMDB-1914 Cite Cite: data citing same article
Similar strucutres (beta)	Diagram EMDB-1734 EMDB-1424 List of similar structure data about Omokage system

Article
Citation - Primary
Article	J. Mol. Biol., Vol. 378, Issue 2, Page 362-74, Year 2008
Title	Structural dynamics of archaeal small heat shock proteins.
Authors	Martin Haslbeck, Andreas Kastenmüller, Johannes Buchner, Sevil Weinkauf, Nathalie Braun Munich Center for Integrated Protein Science (CIPSM), Technische Universität München, Department Chemistry, Lichtenbergstr. 4, 85747 Garching, Germany.
Keywords	Archaeal Proteins (chemistry), Archaeoglobus fulgidus, HSP16.5 protein, Methanococcus jannaschii, HSP20 Heat-Shock Proteins (chemistry), Heat-Shock Proteins (chemistry), Hot Temperature, Microscopy, Electron, Protein Conformation
Links	PII: S0022-2836(08)00171-X, DOI: 10.1016/j.jmb.2008.01.095, PubMed: 18353362

File

Projections & Slices

Size of images:

Images are generated by Spider package.

Density

Data Type

Image stored as Reals

Space Group Number

Map Geometry

Unit Cell

A = 203.52 A , B = 203.52 A , C = 203.52 A ,
alpha = 90 degrees , beta = 90 degrees , gamma = 90 degrees

Pixel Spacing

X = 1.59 A , Y = 1.59 A , Z = 1.59 A

CCP4 map header info

Annotation Details

Small Hsp16.5 assembly

Sample
Name	Hsp16.5 from Methanocaldococcus jannaschii
Number of Components	1
Oligomeric State	24mer
Theoretical Mass	0.396 MDa
Component #1: protein - Small Heat Shock Protein
Scientific name	Small Heat Shock Protein
Theoretical Mass	0.396 MDa
Oligomeric Details	24mer
Scientific Name of Species	Methanocaldococcus jannaschii (NCBI Taxonomy: 2190)
Recombinant expression	Yes
Links	InterPro: IPR:002068, Gene Ontology: GO:0006950

Experiment
Sample Preparation
Staining	Staining with 1.5% (w/v) ammonium molybdate
Specimen Conc	0.1 mg/ml
Specimen Support Details	400 mesh copper grid
Specimen State	particle
Buffer	Details: 50 mM Tris pH: 7.0
Vitrification
Cryogen Name	NONE
Instrument	NONE
Imaging
Microscope	JEOL 100CX
Date	10-MAY-2007
Electron Gun
Electron Source	TUNGSTEN HAIRPIN
Accelerating Voltage	100 kV
Illumination Mode	FLOOD BEAM
Lens
Magnification	Nominal: 50000 X,
Astigmatism	objective lens astigmatism was corrected at 160,000 times magnification
Imaging Mode	BRIGHT FIELD
Defocus	600 nm - 900 nm
Specimen Holder
Holder	Eucentric ( JEOL )
Camera
Detector	Mago-EM23
Image Acquisition
Scanner	OTHER
Sampling Size	7.9 microns
Quant Bit Number	16

Processing
Method	single particle reconstruction
3 D reconstruction
Software	IMAGIC
CTF Correction	whole image
Resolution By Author	19
Resolution Method	FSC at 0.5 cut-off

Data from EMDB

Header (meta data in XML format)

Map data

Images

FTP directory

Movie files

movie #1

	.mp4 (H.264/MPEG-4 AVC format), 3.6 MB .webm (WebM/VP8 format), 5.5 MB Session file for UCSF-Chimera, 26.3 KB

movie #2

	.mp4 (H.264/MPEG-4 AVC format), 3.2 MB .webm (WebM/VP8 format), 5 MB Session file for UCSF-Chimera, 26.3 KB