Summary Database / ID EM DATA BANK (EMDB) / 1900 Authors Goulet A , Lai-Kee-Him J , Veesler D , Auzat I , Robin G , Shepherd DA , Ashcroft AE , Richard E , Lichiere J , Tavares P , Cambillau C , Bron P EMDB Sites Structure Visualization Movies X Movie Page #1 #2 #1 : Surface view with section colored by density value, Surface level: 60, Made by UCSF CHIMERA
#2 : Surface view colored by cylindrical radius, Surface level: 60, Made by UCSF CHIMERA
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Structure viewers (About PeppeR) , RCSB , PDBe )Related Structure Data Similar strucutres (beta)
Citation - Primary Article J. Biol. Chem. , Vol. 286, Issue 28, Page 25397-405, Year 2011Title The opening of the SPP1 bacteriophage tail, a prevalent mechanism in Gram-positive-infecting siphophages. Authors Adeline Goulet , Joséphine Lai-Kee-Him , David Veesler , Isabelle Auzat , Gautier Robin , Dale A Shepherd , Alison E Ashcroft , Eric Richard , Julie Lichière , Paulo Tavares , Christian Cambillau , Patrick Bron Keywords Links
File Projections & Slices Size of images: Axes X SurfaceX X X X
Images are generated by Spider package .
Density
Contour Level: 60 (by author) , 60 (movie #1): Minimum - Maximum: 0 - 100 Average (Standard dev.): 51.7892 (3.06874)
Data Type Image stored as Reals Space Group Number 1 Map Geometry Axis Order : X Y Z Dimensions : 100 100 100 Origin : -50 -49 -50 Limit : 49 50 49 Spacing : 100 100 100
Unit Cell A = 438 A , B = 438 A , C = 438 A , 90 degrees , beta = 90 degrees , gamma = 90 degreesPixel Spacing X = 4.38 A , Y = 4.38 A , Z = 4.38 ACCP4 map header info Show mode Image stored as Reals A/pix X/Y/Z 4.38 4.38 4.38 M x/y/z 100 100 100 origin x/y/z 0.000 0.000 0.000 length x/y/z 438.000 438.000 438.000 alpha/beta/gamma 90.000 90.000 90.000 start NX/NY/NZ -56 -56 -55 NX/NY/NZ 112 112 112 MAP C/R/S 1 2 3 start NC/NR/NS -50 -49 -50 NC/NR/NS 100 100 100 start NC,NX/NR,NY/NS,NZ NC,NX/NR,NY/NS,NZ D min/max/mean 0.000 100.000 51.789
Annotation Details CCP4 Map file of Bacteriophage spp1 Dit-Tal complex
Sample Preparation Specimen Conc 0.07 mg/ml Specimen Support Details Quantifoil R 2/2 grids Specimen State particle Buffer Details: 10 mM HEPES, 150 mM NaCl pH: 7.5 Vitrification Method Blot for 2s, both sides Cryogen Name ETHANE Details Vitrification instrument: Cryoplunge CP3 gatan Humidity 90 Instrument GATAN CRYOPLUNGE 3 Temperature 102.15 Kelvin Imaging Microscope JEOL 2200FS Details Images recorded on a JEOL 2200FS Electron Gun Electron Source FIELD EMISSION GUN Accelerating Voltage 200 kV Electron Dose 20 e/A**2 Illumination Mode FLOOD BEAM Lens Magnification Nominal: 50000 X, Calibrated: 45591 X Astigmatism Objective lens astigmatism was corrected at 200,000 times magnification Nominal Cs 2.0 mm Imaging Mode BRIGHT FIELD Defocus 1400 nm - 2500 nm Energy Filter Type: Omega JEOL , Window: 0-20 eVSpecimen Holder Holder Eucentric ( SIDE ENTRY, EUCENTRIC ) Temperature 98.15 Kelvin Camera Detector Kodak SO163 Image Acquisition Scanner NIKON SUPER COOLSCAN 9000 Number of Digital Images 100 Sampling Size 10 microns
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EMD-1900.map ( map file in CCP4 format, 4002 KB )
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emd-1900.jpg (75.2 KB)
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