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Structure of bacteriophage N4
by single particle reconstruction, at 29 A resolution
#1: Surface view with section colored by density value, Surface level: 1.5, Made by UCSF CHIMERA
#2: Surface view colored by radius, Surface level: 1.5, Made by UCSF CHIMERA
 Entry | |
| Summary | |
| Database / ID | EM DATA BANK (EMDB) / 1475 |
|---|---|
| Title | Structure of bacteriophage N4 |
| Map | This is an asymmetric reconstruction of bacteriophage N4 |
| Sample | Bacteriophage N4 |
| Keywords |  Bacteriophage N4, Podoviridae, non-contractile tail sheath, tail tube, appendages |
| Authors | Choi KH, McPartland J, Kaganman I, Bowman VD, Rothman-Denes LB, Rossmann MG |
| Date | Deposition: 2008-02-22, Header release: 2008-02-25, Map release: 2009-03-31, Last update: 2009-05-05 |
| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
| Structure Visualization | |
| Movies |  Movie Page#1: Surface view with section colored by density value, Surface level: 1.5, Made by UCSF CHIMERA #2: Surface view colored by radius, Surface level: 1.5, Made by UCSF CHIMERA |
| Supplemental images | |
| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
| Related Structure Data | |
| Related Entries |
Cite: data citing same article |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - Primary | |
| Article | J. Mol. Biol., Vol. 378, Issue 3, Page 726-36, Year 2008 |
|---|---|
| Title | Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. |
| Authors | Kyung H Choi, Jennifer McPartland, Irene Kaganman, Valorie D Bowman, Lucia B Rothman-Denes, Michael G Rossmann Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX 77555-0647, USA. |
| Keywords | Bacteriophage N4 (enzymology), Cryoelectron Microscopy, DNA, Viral (metabolism), DNA-Directed RNA Polymerases (metabolism, 2.7.7.6), Protein Transport, Viral Proteins (metabolism) |
| Links | PII: S0022-2836(08)00258-1, DOI: 10.1016/j.jmb.2008.02.059, PubMed: 18374942, PMC: PMC2396777 |
 Map | |||||||||||||||||||||||||
| File |  EMD-1475.map ( map file in CCP4 format, 296354 KB ) | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices | Size of images:
Images are generated by Spider package. | ||||||||||||||||||||||||
| Density |
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| Data Type | Image stored as Reals | ||||||||||||||||||||||||
| Space Group Number | 1 | ||||||||||||||||||||||||
| Map Geometry |
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| Unit Cell | A= B= C: 1486.8 A Alpha=beta=gamma: 90 degrees | ||||||||||||||||||||||||
| Pixel Spacing | X= Y= Z: 3.54 A | ||||||||||||||||||||||||
| CCP4 map header info | |||||||||||||||||||||||||
| Annotation Details | This is an asymmetric reconstruction of bacteriophage N4 | ||||||||||||||||||||||||
 Supplement | |
| Images | |
| Images | |
|---|---|
 Sample | |
| Name | Bacteriophage N4 |
|---|---|
| Number of Components | 10 |
| Component #1: virus - bacteriophage N4 | |
| Scientific name | Enterobacteria phage N4 |
| Common Name | bacteriophage N4 |
| Scientific Name of Species | Enterobacteria phage N4 |
| Common Name of Species | bacteriophage N4 |
| NCBI taxonomy | 10752 |
| Empty | No |
| Enveloped | No |
| Isolate | STRAIN |
| Class | VIRION |
| Natural Source | Host Category: BACTERIA(EUBACTERIA) |
| Shell | Id: 1 , Diameter: 740 A, T Number: 9 |
 Experiment | |
| Sample Preparation | |
| Specimen State | particle |
|---|---|
| Vitrification | |
| Cryogen Name | ETHANE |
| Instrument | NONE |
| Imaging | |
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | |
| Electron Source | FIELD EMISSION GUN |
| Accelerating Voltage | 200 kV |
| Illumination Mode | FLOOD BEAM |
| Lens | |
| Magnification | Nominal: 39500 |
| Imaging Mode | BRIGHT FIELD |
| Defocus | 1130 nm - 3750 nm |
| Specimen Holder | |
| Holder | eucentric |
| Model | GATAN LIQUID NITROGEN |
| Camera | |
| Image Acquisition | |
| Scanner | ZEISS SCAI |
| Sampling Size | 14 |
 Processing | |
| Method | single particle reconstruction |
|---|---|
| 3D reconstruction | |
| Resolution By Author | 29 A |
| Resolution Method | fsc at 0.5 cutoff |
| Single Particle | |
| Number of Projections | 2489 |
| Download | |||
| Data from EMDB | |||
| Header (meta data in XML format) | emd-1475.xml (6 KB) | ||
|---|---|---|---|
| Map data | emd_1475.map.gz (205 MB) | ||
| Images |  1475.gif (92.9 KB) | ||
| FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1475 | ||
| Movie files | |||
| movie #1 |
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| movie #2 |
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