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Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes.
by single particle reconstruction, at 45 A resolution
#1: Surface view with section colored by density value, Surface level: 0.065, Made by UCSF CHIMERA
#2: Surface view colored by radius, Surface level: 0.065, Made by UCSF CHIMERA
 Entry | |
| Summary | |
| Database / ID | EM DATA BANK (EMDB) / 1325 |
|---|---|
| Title | Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes. |
| Map | Human spliceosomal A complex |
| Sample | Human prespliceosomal A Complex |
| Authors | Behzadnia N, Golas MM, Hartmuth K, Sander B, Kastner B, Deckert J, Dube P, Will CL, Urlaub H, Stark H, Luhrmann R |
| Date | Deposition: 2007-02-16, Header release: 2007-02-16, Map release: 2009-02-16, Last update: 2012-10-10 |
| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
| Structure Visualization | |
| Movies |  Movie Page#1: Surface view with section colored by density value, Surface level: 0.065, Made by UCSF CHIMERA #2: Surface view colored by radius, Surface level: 0.065, Made by UCSF CHIMERA |
| Supplemental images | |
| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
| Related Structure Data | |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - Primary | |
| Article | EMBO J., Vol. 26, Issue 6, Page 1737-48, Year 2007 |
|---|---|
| Title | Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes. |
| Authors | Nastaran Behzadnia, Monika M Golas, Klaus Hartmuth, Bjoern Sander, Berthold Kastner, Jochen Deckert, Prakash Dube, Cindy L Will, Henning Urlaub, Holger Stark, Reinhard Lührmann Department of Cellular Biochemistry, MPI of Biophysical Chemistry, Göttingen, Germany. |
| Keywords |  Base Pairing (genetics), Humans, Immunoblotting, Immunoprecipitation, Mass Spectrometry, Microscopy, Electron, Models, Molecular, Oligonucleotides, Proteins (analysis), RNA Precursors (chemistry), Spliceosomes (chemistry), Tobramycin (32986-56-4) |
| Links | DOI: 10.1038/sj.emboj.7601631, PubMed: 17332742, PMC: PMC1829389 |
 Map | |||||||||||||||||||||||||
| File |  EMD-1325.map ( map file in CCP4 format, 4001 KB ) | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections & Slices | Size of images:
Images are generated by Spider package. | ||||||||||||||||||||||||
| Density |
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| Data Type | float (32-bit) | ||||||||||||||||||||||||
| Space Group Number | 1 | ||||||||||||||||||||||||
| Map Geometry |
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| Unit Cell | A= B= C: 490 A Alpha=beta=gamma: 90 degrees | ||||||||||||||||||||||||
| Pixel Spacing | X= Y= Z: 4.9 A | ||||||||||||||||||||||||
| CCP4 map header info | |||||||||||||||||||||||||
| Annotation Details | Human spliceosomal A complex | ||||||||||||||||||||||||
 Supplement | |
| Images | |
| Images | |
|---|---|
 Sample | |
| Name | Human prespliceosomal A Complex |
|---|---|
| Number of Components | 1 |
| Component #1: cellular-component - Prespliceosome | |
| Scientific name | Human prespliceosomal A Complex |
| Common Name | Prespliceosome |
| Oligomeric Details | monomer |
| Scientific Name of Species | Homo sapiens |
| Common Name of Species | Human |
| NCBI taxonomy | 9606 |
| Recombinant expression | No |
| Natural Source | Organelle: nucleus Cell: HeLa |
 Experiment | |
| Sample Preparation | |
| Specimen State | particle |
|---|---|
| Vitrification | |
| Cryogen Name | ETHANE |
| Imaging | |
| Microscope | FEI/PHILIPS CM200FEG |
| Electron Gun | |
| Electron Source | FIELD EMISSION GUN |
| Accelerating Voltage | 200 kV |
| Illumination Mode | SPOT SCAN |
| Lens | |
| Magnification | Nominal: 88000, Calibrated: 122000 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder | |
| Model | OTHER |
| Tilt Angle | 0 degrees - 45 degrees |
| Camera | |
| Detector | TVIPS TemCam F415 |
 Processing | |
| Method | single particle reconstruction |
|---|---|
| 3D reconstruction | |
| Algorithm | RCT |
| Software | IMAGIC |
| Resolution By Author | 45 A |
| Resolution Method | FSC |
| Download | |||
| Data from EMDB | |||
| Header (meta data in XML format) | emd-1325.xml (5.8 KB) | ||
|---|---|---|---|
| Map data | emd_1325.map.gz (2 MB) | ||
| Images |  1325.png (99.9 KB) | ||
| FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1325 | ||
| Movie files | |||
| movie #1 |
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| movie #2 |
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