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Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26.
by single particle reconstruction, at 14.2 A resolution
#1: Surface view with section colored by density value, Surface level: 0.035471745, Made by UCSF CHIMERA
#2: Surface view colored by radius, Surface level: 0.035471745, Made by UCSF CHIMERA
 Entry | |
| Summary | |
| Database / ID | EM DATA BANK (EMDB) / 1227 |
|---|---|
| Title | Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. |
| Map | Surface view of the compact form of a variant form of yeast Hsp26 |
| Sample | Hsp26, DeltaN1-30 truncation |
| Authors | White HE, Orlova EV, Chen S, Wang L, Ignatiou A, Gowen B, Stromer T, Franzmann TM, Haslbeck M, Buchner J, Saibil HR |
| Date | Deposition: 2006-05-09, Header release: 2006-05-24, Map release: 2006-07-25, Last update: 2011-05-26 |
| EMDB Sites |  EMDB @PDBe (EU), EMDB @RCSB (USA) |
| Structure Visualization | |
| Movies |  Movie Page#1: Surface view with section colored by density value, Surface level: 0.035471745, Made by UCSF CHIMERA #2: Surface view colored by radius, Surface level: 0.035471745, Made by UCSF CHIMERA |
| Supplemental images | |
| Structure viewers |  Yorodumi,  Launch PeppeR (About PeppeR), Volume viewer (RCSB, PDBe) |
| Related Structure Data | |
| Related Entries |
Cite: data citing same article |
| Similar strucutres (beta) |
 List of similar structure data  about Omokage system |
 Article | |
| Citation - Primary | |
| Article | Structure, Vol. 14, Issue 7, Page 1197-204, Year 2006 |
|---|---|
| Title | Multiple distinct assemblies reveal conformational flexibility in the small heat shock protein Hsp26. |
| Authors | Helen E White, Elena V Orlova, Shaoxia Chen, Luchun Wang, Athanasios Ignatiou, Brent Gowen, Thusnelda Stromer, Titus M Franzmann, Martin Haslbeck, Johannes Buchner, Helen R Saibil Department of Crystallography, Birkbeck College, London WC1E 7HX, United Kingdom. |
| Keywords |  Binding Sites, Cryoelectron Microscopy, Dimerization, HSP26 protein, S cerevisiae, Heat-Shock Proteins (chemistry), Protein Structure, Quaternary, Saccharomyces cerevisiae Proteins (chemistry), alpha-Crystallins (chemistry) |
| Links | PII: S0969-2126(06)00260-7, DOI: 10.1016/j.str.2006.05.021, PubMed: 16843901 |
 Map | |||||||||||||||||||||||||
| File |  EMD-1227.map ( map file in CCP4 format, 8389 KB ) | ||||||||||||||||||||||||
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| Projections & Slices | Size of images:
Images are generated by Spider package. | ||||||||||||||||||||||||
| Density |
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| Data Type | float (32-bit) | ||||||||||||||||||||||||
| Space Group Number | 1 | ||||||||||||||||||||||||
| Map Geometry |
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| Unit Cell | A = 238.08 A , B = 238.08 A , C = 238.08 A , alpha = 90 degrees , beta = 90 degrees , gamma = 90 degrees | ||||||||||||||||||||||||
| Pixel Spacing | X = 1.86 A , Y = 1.86 A , Z = 1.86 A | ||||||||||||||||||||||||
| CCP4 map header info | |||||||||||||||||||||||||
| Annotation Details | Surface view of the compact form of a variant form of yeast Hsp26 | ||||||||||||||||||||||||
 Supplement | |
| Images | |
| Images | |
|---|---|
 Sample | |
| Name | Hsp26, DeltaN1-30 truncation |
|---|---|
| Oligomeric State | 24-mer |
| Number of Components | 1 |
| Component #1: protein - Hsp26 | |
| Scientific name | Hsp26 |
| Oligomeric Details | 24-mer |
| Number of Copies | 1 |
| Scientific Name of Species | Saccharomyces cerevisiae (NCBI Taxonomy: 4932)
|
| Common Name of Species | yeast |
| Recombinant expression | Yes |
| Engineered Source | Exp System: Saccharomyces cerevisiae (NCBI Taxonomy: 4932) |
 Experiment | |
| Sample Preparation | |
| Specimen Conc | 0.7 mg/ml |
|---|---|
| Specimen State | particle |
| Buffer | Details: 20 mM Hepes/KOH, 50 mM KCl, 5 mM EDTA, 1 mM DTT pH: 7.5 |
| Vitrification | |
| Cryogen Name | ETHANE |
| Imaging | |
| Microscope | FEI TECNAI F20 |
| Electron Gun | |
| Electron Source | FIELD EMISSION GUN |
| Accelerating Voltage | 200 kV |
| Illumination Mode | FLOOD BEAM |
| Lens | |
| Magnification | Nominal: 50000 X, |
| Imaging Mode | BRIGHT FIELD |
| Defocus | 1700 nm - 3400 nm |
| Specimen Holder | |
| Holder | Eucentric ( GATAN LIQUID NITROGEN ) |
| Camera | |
| Image Acquisition | |
| Number of Digital Images | 68 |
| Sampling Size | 1.4 microns |
| Scanner | ZEISS SCAI |
 Processing | |
| Method | single particle reconstruction |
|---|---|
| 3 D reconstruction | |
| Algorithm | Angular reconstitution |
| Software | Imagic |
| CTF Correction | Phase flipping |
| Resolution By Author | 14.2 |
| Resolution Method | FSC at 0.5 cut-off |
| Details | Angular reconstitution |
| Single Particle | |
| Number of Class Averages | 625 |
| Number of Projections | 4300 |
| Atomic Model Fitting | |
| Model #0 | |
| Refinement Protocol | rigid body |
| Software | URO |
| Refinement Space | RECIPROCAL |
| Details | Protocol: Rigid Body. The alpha crystallin domain dimers were fitted manually into the same region of density observed in the WT maps prior to refinement in URO |
| Download | |||
| Data from EMDB | |||
| Header (meta data in XML format) | emd-1227.xml (6.8 KB) | ||
|---|---|---|---|
| Map data | emd_1227.map.gz (781.6 KB) | ||
| Images |  1227.gif (57.3 KB) | ||
| FTP directory | ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1227 | ||
| Movie files | |||
| movie #1 |
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| movie #2 |
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